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biotin anti human cd25 goat polyclonal antibody  (R&D Systems)


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    R&D Systems biotin anti human cd25 goat polyclonal antibody
    Biotin Anti Human Cd25 Goat Polyclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+human+cd25+polyclonal+antibody/Human+CD25%2FIL-2R+alpha+Biotinylated+Antibody/pm41308987-150-31-36
    Average 94 stars, based on 12 article reviews
    biotin anti human cd25 goat polyclonal antibody - by Bioz Stars, 2026-10
    94/100 stars

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    Recombinant:

    Article Title: A simplified direct on-chip forward or reverse immunoassay for evaluating protein-protein interactions in the serum.
    Article Snippet: 1-(3-Dimethylamino)propyl)-N-ethyl carbodiimide hydrochloride (EDC), bovine serum albumin (BSA), phosphate buffer saline (PBS, 0.01 M, pH 7.4), N-hydroxysuccinimide (NHS), sodium chloride (NaCl), sodium hydroxide (NaOH), acetic acid, and hydrochloric acid (HCl) were purchased from Sigma–Aldrich (USA). .. Goat anti-human CD25 polyclonal antibody (pAb; BAF223), recombinant human CD25 (223-2A), and recombinant human IL-2 (202-IL) were obtained from R&D Systems (Minneapolis, MN, USA). .. Alexa Fluor@647 labeled anti-human IL-2 monoclonal antibody (mAb; ab199215) was supplied by Abcam (Shanghai, China).



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    Multiple allogeneic mesenchymal stem cell (MSC) injections result in changes in splenic regulatory T cell percentages. (A-D) There were no significant changes in splenic CD21 + B-cell (A) , CD4 + T-cell (B) , or CD8 + T-cell percentages (C) or CD4/CD8 ratios (D) following multiple MSC injections. (E) There were no significant changes in activated <t>(CD25</t> + ) lymphocyte proportions. (F) There were significantly higher percentages of splenic FoxP3 + regulatory T cells in the horses injected with bone marrow (BM)-derived MSCs compared with horses injected with adipose tissue (AT)-derived MSCs. Data are presented as mean ± standard error of the mean. * P <0.05.
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    Multiple allogeneic mesenchymal stem cell (MSC) injections result in changes in splenic regulatory T cell percentages. (A-D) There were no significant changes in splenic CD21 + B-cell (A) , CD4 + T-cell (B) , or CD8 + T-cell percentages (C) or CD4/CD8 ratios (D) following multiple MSC injections. (E) There were no significant changes in activated (CD25 + ) lymphocyte proportions. (F) There were significantly higher percentages of splenic FoxP3 + regulatory T cells in the horses injected with bone marrow (BM)-derived MSCs compared with horses injected with adipose tissue (AT)-derived MSCs. Data are presented as mean ± standard error of the mean. * P <0.05.

    Journal: Stem Cell Research & Therapy

    Article Title: Multiple intravenous injections of allogeneic equine mesenchymal stem cells do not induce a systemic inflammatory response but do alter lymphocyte subsets in healthy horses

    doi: 10.1186/s13287-015-0050-0

    Figure Lengend Snippet: Multiple allogeneic mesenchymal stem cell (MSC) injections result in changes in splenic regulatory T cell percentages. (A-D) There were no significant changes in splenic CD21 + B-cell (A) , CD4 + T-cell (B) , or CD8 + T-cell percentages (C) or CD4/CD8 ratios (D) following multiple MSC injections. (E) There were no significant changes in activated (CD25 + ) lymphocyte proportions. (F) There were significantly higher percentages of splenic FoxP3 + regulatory T cells in the horses injected with bone marrow (BM)-derived MSCs compared with horses injected with adipose tissue (AT)-derived MSCs. Data are presented as mean ± standard error of the mean. * P <0.05.

    Article Snippet: The following antibodies were used: mouse-anti-equine CD3 (clone UC F6G 1:250; Jeffery Stott, University of California, Davis, CA, USA) [ ], mouse-anti-human CD21 (clone B-ly4 1:20; BD Pharmingen, San Jose, CA, USA) [ , ], polyclonal goat-anti-human CD25 (clone AF-223; R&D Systems) [ ], rat-anti-mouse/human FoxP3 (clone PCH101; ebioscience, San Diego, CA, USA) [ ], mouse-anti-CD4 (clone HB61A 1:133; VMRD, Pullman, WA, USA) [ ], mouse-anti-equine CD8 (clone F18P 1:500; J. Stott) [ ], and a donkey-anti-mouse secondary when necessary (1:50; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA).

    Techniques: Injection, Derivative Assay